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Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells stained for NDUFS1 rabbit pAb (12444-1-AP) at dilution of 1:50 and alpha Tubulin mouse mAb (66031-1-Ig) at dilution of 1:100, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 12444-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-mouse IgG (H+L) for 66031-1-Ig.
The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.